IPO8 Polyclonal antibody

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  WB analysis using 32476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32476-1-AP (IPO8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IPO8 Polyclonal Antibody for WB, ELISA

Cat No. 32476-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Imp8, Importin-8, Ran-binding protein 8, RanBP8

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, MDA-MB-231 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32476-1-AP targets IPO8 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPO8 fusion protein Ag35395
• Full Name: importin 8
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC167853
• Gene Symbol: IPO8
• Gene ID (NCBI): 10526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O15397
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IPO8(Importin 8) is a gene encoding protein, which is located on human chromosome 12. It belongs to the nuclear protein transport family, and is mainly involved in the process of nuclear protein introduction. IPO8 regulates the transport function of nuclear pore complex by interacting with Ran GTPase and other proteins (PMID: 11682607). It is expected to be located in the cytoplasm and nucleus. IPO8 is associated with many diseases, including VISS syndrome and Loeys-Dietz syndrome. These diseases usually involve cardiovascular defects, skeletal abnormalities and immune loss. The molecular weight of PO8 protein is about 125kDa, and its structure includes helix and β-sheet. It has a specific three-dimensional structure and can form complexes with other protein.