IQCD Polyclonal antibody

• 

  WB analysis of mouse testis using 26345-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26345-1-AP (IQCD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26345-1-AP (IQCD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IQCD Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26345-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DRC10, Dynein regulatory complex protein 10, IQ domain containing protein D, IQ domain-containing protein D, IQ motif containing D

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26345-1-AP targets IQCD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23692

  Product name: Recombinant human IQCD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-347 aa of BC013151

  Sequence: MALDILAMAPLYQAPAINRIGPKTDPSKRPADPLKPLVLSRTKLTTIEAKRIMSILDEAIYKVELVTLLSYVASNREDMEGMLGEDVMRAVREHEDLCQVLLENVRCLKEKERQLQEQKEAEEEGWLRDRLLSIELQKSSLSPLMQQIKDSTKNVLRLLLSNPQAARLLQMQTQGRSAEAQNFIDSLIELRGFLFEKLLTSPMEARDKAQFLQDISRQNSNNQQIIDTLEKELAERMKNRNAEEELEDLDAVHREEKISLEELRRRHKVLVGEFAQIREEREINSKKRMEAEQEMVRMVRAATLIQALWKGYLVRSLLRSKKKRGKGKAKDKEKGKQKGKEKGKGKK

• Full Name: IQ motif containing D
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC013151
• Gene Symbol: IQCD
• Gene ID (NCBI): 115811
• RRID: AB_2880485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96DY2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQCD has some isoforms with MW 52 kDa, 37-40 kDa. All these isoforms can be detected by this antibody.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC,IF	Reprod Fertil Dev; Corrigendum to: IQ motif containing D (IQCD), a new acrosomal protein involved in the acrosome reaction and fertilisation.; Authors - Peng Zhang