Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26345-1-AP targets IQCD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23692 Product name: Recombinant human IQCD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC013151 Sequence: MALDILAMAPLYQAPAINRIGPKTDPSKRPADPLKPLVLSRTKLTTIEAKRIMSILDEAIYKVELVTLLSYVASNREDMEGMLGEDVMRAVREHEDLCQVLLENVRCLKEKERQLQEQKEAEEEGWLRDRLLSIELQKSSLSPLMQQIKDSTKNVLRLLLSNPQAARLLQMQTQGRSAEAQNFIDSLIELRGFLFEKLLTSPMEARDKAQFLQDISRQNSNNQQIIDTLEKELAERMKNRNAEEELEDLDAVHREEKISLEELRRRHKVLVGEFAQIREEREINSKKRMEAEQEMVRMVRAATLIQALWKGYLVRSLLRSKKKRGKGKAKDKEKGKQKGKEKGKGKK Predict reactive species |
| Full Name | IQ motif containing D |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC013151 |
| Gene Symbol | IQCD |
| Gene ID (NCBI) | 115811 |
| RRID | AB_2880485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IQCD has some isoforms with MW 52 kDa, 37-40 kDa. All these isoforms can be detected by this antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IQCD antibody 26345-1-AP | Download protocol |
| WB protocol for IQCD antibody 26345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IQCD Polyclonal antibody (Cat# 26345-1-AP) has 1 total citation. It appears in Reproduction, Fertility and Development (IF 2.3), in a corrigendum describing IQCD as a novel acrosomal protein involved in the acrosome reaction and fertilization. The research field is reproductive biology, specifically male gamete function. The cited applications include WB, IHC, and IF, with reactivity in both human and mouse.
| Species | Application | Title |
|---|---|---|
Reprod Fertil Dev Corrigendum to: IQ motif containing D (IQCD), a new acrosomal protein involved in the acrosome reaction and fertilisation. |



