IQCG Polyclonal antibody

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• IQCG Polyclonal antibody (33459-1-AP)

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  WB analysis using 33459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33459-1-AP (IQCG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IQCG Polyclonal Antibody for WB, ELISA

Cat No. 33459-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DRC9, Dynein regulatory complex protein 9, IQ domain-containing protein G

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33459-1-AP targets IQCG in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37506

  Product name: Recombinant human IQCG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-372 aa of BC004816

  Sequence: TDQLSILNYIMPVQYEGRQSICVKSREMNLEGTNLDKLPMDSTITKIPSPLITEEGPNLPEIRHRGRFAVEFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSHVIADTIKELQDSATYNSLLQALSKERENKMHFYDIIAREEKGRKQIISLQKQLINVKKEWQFEVQSQNEYIANLKDQLQEMKAKSNLENRYMKTNTELQIAQTQKKCNRTEELLVEEIEKLRMKTEEEARTHTEIEMFLRKEQQKLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAHLQDLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVIKLQAWWRGTMIRREIGGFKMPKDKVDSKDSKGKGKGKDKRRGKKK

• Full Name: IQ motif containing G
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004816
• Gene Symbol: IQCG
• Gene ID (NCBI): 84223
• RRID: AB_3742980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H095
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human IQ motif containing G (IQCG) gene was first reported to be involved in the chromosome translocation in a case of acute T-lymphoid/myeloid leukemia. The expression of Iqcg is prominently enriched in the testis of mice and reveals an ordered expression pattern during spermatogenesis. Iqcg knockout (KO) mice are sterile, due to the severe malformation and total immobility of their spermatozoa. The axoneme in the KO sperm flagellum is disorganized and hardly any typical normal "9+2" pattern, composed of two central microtubules surrounded by nine microtubular doublets, could be found in the KO spermatids. However, the cilia structures in the trachea and oviduct, as well as histological appearances of other major tissues, remain unaffected in the KO mice, suggesting Iqcg's specific role in spermiogenesis (PMID: 24849454).