Tested Applications
| Positive WB detected in | HepG2 cells, human peripheral blood platelets |
| Positive IHC detected in | human liver cancer tissue, human pancreas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55189-1-AP targets IQGAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | IQ motif containing GTPase activating protein 2 |
| Calculated Molecular Weight | 181 kDa |
| Observed Molecular Weight | 181 kDa, 65 kDa |
| GenBank Accession Number | NM_006633 |
| Gene Symbol | IQGAP2 |
| Gene ID (NCBI) | 10788 |
| RRID | AB_2881286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IQGAP2 antibody 55189-1-AP | Download protocol |
| WB protocol for IQGAP2 antibody 55189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IQGAP1 antibody (Cat# 55189-1-AP) has 5 total citations published in Cell Death & Disease, BMC Cancer, Biochimica et Biophysica Acta – General Subjects, Human Cell, and Medicine (Baltimore). The associated research fields span breast cancer migration, hepatocellular carcinoma prognosis, DP1 receptor signaling and trafficking, PI3K-mediated cell proliferation, and clear cell renal cell carcinoma immunometabolism. Applications used include WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration
| ||
BMC Cancer Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma | ||
Biochim Biophys Acta Gen Subj Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.
| ||
Hum Cell Upstream regulatory mechanisms and clinical significance of IQGAP2 deficiency in affecting PI3K activity to drive cell proliferation.
| ||
Medicine (Baltimore) Comprehensive investigation of a new signature integrating lipid metabolism and immune-related genes for clear cell renal cell carcinoma prognosis and immunometabolism landscapes. |
Reviews
What customers say
The single review for this product is a 5-star rating, with the reviewer noting that it "works as it should be" for Western Blot applications. Overall, the feedback is positive, indicating reliable performance for its intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ziwei (Verified Customer) (01-04-2026) | works as it should be.
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