IQGAP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IQGAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55189-1-AP

Synonyms

IQGAP2

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Validation Data Gallery

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  WB analysis using 55189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55189-1-AP (IQGAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55189-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55189-1-AP (IQGAP2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, human peripheral blood platelets
• Positive IHC detected in: human liver cancer tissue, human pancreas tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

55189-1-AP targets IQGAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IQ motif containing GTPase activating protein 2
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 181 kDa, 65 kDa
• GenBank Accession Number: NM_006633
• Gene Symbol: IQGAP2
• Gene ID (NCBI): 10788
• RRID: AB_2881286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma; Authors - Qingqing Dai
human	WB	Biochim Biophys Acta Gen Subj; Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.; Authors - Louis Fréchette ; KD Validated
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu ; KD Validated