Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, A549 cells, HEK-293 cells, HepG2 cells, Calu-1 cells |
| Positive IHC detected in | human placenta tissue, human stomach cancer tissue, rat small intestine tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25930-1-AP targets IQGAP3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23109 Product name: Recombinant human IQGAP3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 530-880 aa of BC131536 Sequence: LINEALDKGSPEKTLSALLLPAAGLDDVSLPVAPRYHLLLVAAKRQKAQVTGDPGAVLWLEEIRQGVVRANQDTNTAQRMALGVAAINQAIKEGKAAQTERVLRNPAVALRGVVPDCANGYQRALESAMAKKQCPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGCPLNTSHLTREEIQSAVTKVTAAYDRQQLWKANVGFVIQLQARLRGFLVRQKFAEHSHFLRTWLPAVIKIQAHWRGYRQRKIYLEWLQYFKANLDAIIKIQAWARMWAARRQYLRRLHYFQKNVNSIVKIQAFFRARKAQDDYRILVHAPHPPLSVVRRFAHLLNQSQQDFLAEAELLKLQEE Predict reactive species |
| Full Name | IQ motif containing GTPase activating protein 3 |
| Calculated Molecular Weight | 1631 aa, 185 kDa |
| Observed Molecular Weight | 185 kDa |
| GenBank Accession Number | BC131536 |
| Gene Symbol | IQGAP3 |
| Gene ID (NCBI) | 128239 |
| RRID | AB_2880299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
| WB protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody targeting IQGAP3 (Cat# 25930-1-AP) has 12 citations published in journals including Cell Death & Disease, Cancer Letters, Clinical Cancer Research, Cell & Molecular Life Sciences, Scientific Reports, American Journal of Pathology, European Journal of Medical Research, iScience, BMC Cancer, Pathology Research & Practice, PeerJ, and Biochimica et Biophysica Acta. Research fields span lung cancer, hepatocellular carcinoma, osteosarcoma, and renal cell carcinoma, covering metastasis, immune response, stemness, drug resistance, and signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction | ||
Cancer Lett IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer. | ||
Clin Cancer Res Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma | ||
Cell Mol Life Sci Targeting IQGAP3 skews macrophages polarization towards M1 phenotype and promotes antitumor immune response. | ||
Sci Rep IQGAP3 activates Hedgehog signaling to confer stemness and metastasis via up-regulating GLI1 in lung cancer
| ||
Am J Pathol Focal Adhesion Kinase Activation Mediates IQ Motif-Containing GTPase-Activating Protein 3-Promoted Lung Metastasis in Osteosarcoma. |
