IQGAP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

IQGAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 25930-1-AP

Synonyms

IQGAP3

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Validation Data Gallery

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  WB analysis using 25930-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25930-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, MCF-7 cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

25930-1-AP targets IQGAP3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IQGAP3 fusion protein Ag23109
• Full Name: IQ motif containing GTPase activating protein 3
• Calculated Molecular Weight: 1631 aa, 185 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC131536
• Gene Symbol: IQGAP3
• Gene ID (NCBI): 128239
• RRID: AB_2880299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.; Authors - Yulan Zeng
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma; Authors - Qingqing Dai
human	WB	Biochim Biophys Acta Gen Subj; Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.; Authors - Louis Fréchette ; KD Validated
human	WB	iScience; Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer; Authors - Khine Myint
human	WB	PeerJ; IQGAP3 in clear cell renal cell carcinoma contributes to drug resistance and genome stability; Authors - Wen Li ; KD Validated
human	WB,IHC,IF	Clin Cancer Res; Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma; Authors - Miaoling Tang