IQSEC2 Polyclonal antibody

IQSEC2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25321-1-AP

Synonyms

BRAG1, IQ motif and Sec7 domain 2, IQSEC2, KIAA0522

---

Validation Data Gallery

• 

  IHC staining of human kidney using 25321-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25321-1-AP (IQSEC2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 25321-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25321-1-AP (IQSEC2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 25321-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25321-1-AP (IQSEC2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 25321-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25321-1-AP (IQSEC2 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive IHC detected in: human kidney tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25321-1-AP is IQSEC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IQSEC2 fusion protein Ag18226
• Full Name: IQ motif and Sec7 domain 2
• Calculated Molecular Weight: 1488 aa, 163 kDa
• GenBank Accession Number: BC156683
• Gene Symbol: IQSEC2
• Gene ID (NCBI): 23096
• RRID: AB_2880030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.