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IRAK1 Monoclonal antibody

IRAK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human and More (1)







Cat no : 66653-1-Ig


IRAK, IRAK 1, IRAK1, pelle

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66653-1-Ig targets IRAK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IRAK1 fusion protein Ag0728
Full Name interleukin-1 receptor-associated kinase 1
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014963
Gene Symbol IRAK1
Gene ID (NCBI) 3654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)


Product Specific Protocols
WB protocol for IRAK1 antibody 66653-1-IgDownload protocol
IHC protocol for IRAK1 antibody 66653-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Mol Immunol

Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation.

Authors - Gang Xu

Cell Death Dis

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells

Authors - Jing Li

Biol Reprod

miR-146b-5p downregulates IRAK1 and ADAM19 to suppress trophoblast proliferation, invasion and migration in miscarriage

Authors - Xiaoli Zhang