IRAK2 Polyclonal antibody

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  WB analysis using 28200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28200-1-AP (IRAK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IRAK2 Polyclonal Antibody for WB, ELISA

Cat No. 28200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Interleukin-1 receptor-associated kinase-like 2, IRAK 2, IRAK-2

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse spleen tissue, rat kidney tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28200-1-AP targets IRAK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRAK2 fusion protein Ag28179
• Full Name: interleukin-1 receptor-associated kinase 2
• Calculated Molecular Weight: 625 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC125184
• Gene Symbol: IRAK2
• Gene ID (NCBI): 3656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43187
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRAK2 (Interleukin-1 receptor-associated kinase 2) is a crucial protein involved in innate immune signaling and has diverse roles in various biological processes. IRAK2 belongs to the IRAK family of proteins, which share an N-terminal death domain (DD) that enables interactions with the DD-containing MyD88, and a central kinase domain (KD) (PMID: 24973222). IRAK2 has been implicated in various cancers. It is highly expressed in pancreatic cancer patients and is associated with poor prognosis. IRAK2 knockdown impairs pancreatic cancer cell proliferation (PMID: 37108068).