Tested Applications
| Positive WB detected in | mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | mouse colon tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28766-1-AP targets IRAK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30309 Product name: Recombinant human IRAK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 368-500 aa of BC057800 Sequence: LTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDR Predict reactive species |
| Full Name | interleukin-1 receptor-associated kinase 3 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC057800 |
| Gene Symbol | IRAK3 |
| Gene ID (NCBI) | 11213 |
| RRID | AB_2881211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y616 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
I'll search for citations of this catalog number.The IRAK3 Polyclonal antibody (Catalog #28766-1-AP) has a total of 3 citations. The citations appear in the journals Pharmacological Research (impact factor 12.2, 2026), Inflammation (impact factor 5.4, 2024), and Journal of Orthopaedic Surgery and Research (impact factor 3.8, 2025). The research fields associated with these citations include gut-microbiota-modulated colitis (via toll-like receptor 2 signaling), sepsis-related neutrophil extracellular trap formation, and osteoarthritis diagnostic/therapeutic target validation via bioinformatics and experimental studies.
| Species | Application | Title |
|---|---|---|
Pharmacol Res Differential toll-like receptor 2 activation by Akkermansia muciniphila and Bacteroides thetaiotaomicron mediates the beneficial effects of Fu brick tea polysaccharide against colitis. | ||
Inflammation CircIRAK3 Promotes Neutrophil Extracellular Trap Formation by Improving the Stability of ELANE mRNA in Sepsis
| ||
J Orthop Surg Res Integrated analysis of bioinformatics, mendelian randomization, and experimental validation reveals novel diagnostic and therapeutic targets for osteoarthritis: progesterone as a potential therapeutic agent |
