IREB2 Polyclonal antibody, PBS Only

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• IREB2 Polyclonal antibody, PBS Only (29976-1-PBS)

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  WB analysis using 29976-1-AP (same clone as 29976-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

IREB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 29976-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

29976-1-PBS targets IREB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32233

  Product name: Recombinant human IREB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 50-255 aa of BC017880

  Sequence: VRNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQI

• Full Name: iron-responsive element binding protein 2
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC017880
• Gene Symbol: IREB2
• Gene ID (NCBI): 3658
• RRID: AB_3086201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48200
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.