IRF1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat no : MP02217-1

Synonyms

interferon regulatory factor 1, IRF 1, IRF-1, MAR

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP02217-1

  Sandwich ELISA standard curve of MP02217-1, Human IRF1 Recombinant Matched Antibody Pair - PBS only. 85254-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1879. 85254-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP02217-1

  The mean IRF1 concentration was determined to be 12.24 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load and 8.45 ng/mL in THP-1 cell extract based on a 3.40 mg/mL extract load.

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Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02217-1 targets IRF1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: IRF1 Recombinant antibody, PBS Only (Capture) 85254-5-PBS (250167G11). 100 μg. Concentration 1 mg/ml.

Detection antibody: IRF1 Recombinant antibody, PBS Only (Detector) 85254-4-PBS (250167C4). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag1879

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 85254-5-PBS
• Tested Reactivity: human
• Immunogen: IRF1 fusion protein Ag1879
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: IRF 1
• Synonyms: interferon regulatory factor 1, IRF 1, IRF-1, MAR
• GenBank accession number: BC009483
• Gene symbol: IRF1
• Uniprot: P10914
• Gene ID (NCBI): 3659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 85254-4-PBS
• Tested Reactivity: human
• Immunogen: IRF1 fusion protein Ag1879
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: IRF 1
• Synonyms: interferon regulatory factor 1, IRF 1, IRF-1, MAR
• GenBank accession number: BC009483
• Gene symbol: IRF1
• Uniprot: P10914
• Gene ID (NCBI): 3659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.