IRF2BP2 Monoclonal antibody

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• IRF2BP2 Monoclonal antibody (61030-1-Ig)

IRF2BP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 61030-1-Ig

Clone No.1H8G10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Interferon regulatory factor 2-binding protein 2, IRF 2BP2, IRF2BP 2

• 

  WB analysis using 61030-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 61030-1-Ig (IRF2BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 61030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRF2BP2 antibody (61030-1-Ig, Clone: 1H8G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 61030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP2 antibody (61030-1-Ig, Clone: 1H8G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-29 cells, LoVo cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells
• Positive IF/ICC detected in: U2OS cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

61030-1-Ig targets IRF2BP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: interferon regulatory factor 2 binding protein 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_182972
• Gene Symbol: IRF2BP2
• Gene ID (NCBI): 359948
• RRID: AB_3743262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z5L9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.