IRF8 Polyclonal antibody

IRF8 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 24437-1-AP

Synonyms

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

---

Validation Data Gallery

• 

  WB analysis of Raji using 24437-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24437-1-AP (IRF8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24437-1-AP is IRF8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF8 fusion protein Ag19909
• Full Name: ICSBP1
• Calculated Molecular Weight: 426 aa, 48 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC126247
• Gene Symbol: IRF8
• Gene ID (NCBI): 3394
• RRID: AB_2879549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRFs comprise a family of transcription factors that function within the Jak/Stat pathway to regulate IFN and IFN-inducible gene expression in response to viral infection. IRFs predominantly express in lymphoid tissues and play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-8/ICSCP is expressed predominately in hematopoietic cells and is further increased upon treatment with IFN (2111015,1460054). IRF-8 can function as a transcription repressor of ICS-containing promoters (1460054). Expression of IRF-8 can lead to the down-regulation of the anti-apoptotic protein Bcl-2 (14656881). Originally described as being induced by IFN-γ, IRF-8 expression is also elevated by IRF-α as well as IL-12 in NK and T cells (14581002). IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of IFNs, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia (9120398). IRF-8 also regulates bone metabolism by suppressing osteoclast formation (19718038).This antibody specifically recognizes the 48kd IRF8 protein.