Tested Applications
| Positive WB detected in | IFN gamma+LPS treated THP-1 cells, LPS treated RAW 264.7 cells |
| Positive IF/ICC detected in | LPS treated RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28436-1-AP targets IRG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28913 Product name: Recombinant human IRG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 285-481 aa of NM_001258406 Sequence: DAAASVRKHLVAERALLPTDYIKRIVLRIPNVQYVNRPFPVSEHEARHSFQYVACAMLLDGGITVPSFHECQINRPQVRELLSKVELEYPPDNLPSFNILYCEISVTLKDGATFTDRSDTFYGHWRKPLSQEDLEEKFRANASKMLSWDTVESLIKIVKNLEDLEDCSVLTTLLKGPSPPEVASNSPACNNSITNLS Predict reactive species |
| Full Name | immunoresponsive 1 homolog (mouse) |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | NM_001258406 |
| Gene Symbol | IRG1 |
| Gene ID (NCBI) | 730249 |
| RRID | AB_2935468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NK06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IRG1 antibody 28436-1-AP | Download protocol |
| WB protocol for IRG1 antibody 28436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IRG1 Polyclonal antibody (Cat# 28436-1-AP) has 12 citations across journals including ACS Nano, Journal of Controlled Release, Cell Communication and Signaling, Apoptosis, Free Radical Biology and Medicine, Journal of Ethnopharmacology, International Immunopharmacology, Journal of Inflammation Research, and Pathology Research & Practice. Research fields span immunometabolism (itaconate/Nrf2 pathway), inflammation, ferroptosis, liver injury, sepsis, osteoporosis, celiac disease, cartilage degeneration, diabetic wound healing, and breast cancer.
| Species | Application | Title |
|---|---|---|
ACS Nano Harnessing Kupffer Cell Metabolic Rewiring: Rapamycin-Gliadin Nanoparticle as a Pivotal Strategy for Immune Tolerance in Celiac Disease
| ||
J Control Release Acid neutralization and metal-phenolic nanomedicine on-demand release microspheres for reversing cartilage degeneration.
| ||
Cell Commun Signal Activity-based chemical proteomics reveals caffeic acid ameliorates pentylenetetrazol-induced seizures by covalently targeting aconitate decarboxylase 1 | ||
Cell Commun Signal IRG1/itaconate enhances efferocytosis by activating Nrf2-TIM4 signaling pathway to alleviate con A induced autoimmune liver injury
| ||
Apoptosis Itaconate promotes mitophagy to inhibit neuronal ferroptosis after subarachnoid hemorrhage | ||
Apoptosis IRG1/Itaconate inhibits proliferation and promotes apoptosis of CD69+CD103+CD8+ tissue-resident memory T cells in autoimmune hepatitis by regulating the JAK3/STAT3/P53 signalling pathway |
Reviews
What customers say
The product received a 5-star rating from a user who tested it for Western Blot at a 1:2000 dilution. The reviewer reported that it works perfectly for HEK cells, performing well even at high dilutions. Overall, the feedback is positive, highlighting strong performance and reliability in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (08-14-2025) | Works perfectly for HEK cells; even highly diluted
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