IRGC Polyclonal antibody

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• IRGC Polyclonal antibody (14090-1-AP)

IRGC Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14090-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

IRGC1, Interferon-inducible GTPase 5, Immunity-related GTPase cinema 1, IIGP5, EC:3.6.5.-

• 

  WB analysis using 14090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14090-1-AP (IRGC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 14090-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using IRGC antibody (14090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14090-1-AP targets IRGC in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5279

  Product name: Recombinant human IRGC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-463 aa of BC066939

  Sequence: MATSKLPVVPGEEENTILMAKERLEALRTAFESGDLPQAASHLQELLASTESIRLEVGVTGESGAGKSSLINALRGLEAEDPGAALTGVMETTMQPSPYPHPQFPDVTLWDLPGAGSPGCPADKYLKQVDFSRYDFFLLVSPRRCGAVETRLAAEILCQGKKFYFVRTKVDEDLAATRTQRPSGFREAAVLQEIRDHCAERLREAGVADPRIFLVSNLSPARYDFPTLVSTWEHDLPSHRRHAGLLSLPDISLEALQKKKAMLQEQVLKTALVLGVIQALPVPGLAAAYDDALLIHSLRGYHRSFGLDDDSLAKLAEQVGKQAGDLRSVIRSPLANEVSPETVLRLYSQSSDGAMRVARAFERGIPVFGTLVAGGISFGAVYTMLQGCLNEMAEDAQRVRIKALEDDEPQPEVSLEVASDNGVEKGGSGEGGGEEAPLSTCRKLGLLLKYILDSWKKHDSEEK

• Full Name: immunity-related GTPase family, cinema
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC066939
• Gene Symbol: IRGC
• Gene ID (NCBI): 56269
• RRID: AB_2127712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NXR0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The IRGC polyclonal antibody (Cat# 14090-1-AP) has 2 total citations. These appear in Frontiers in Cardiovascular Medicine and Developmental Biology. The cited research spans cardiovascular biology, examining SFRP5's role in protecting against cardiac rupture via mitochondrial dysfunction inhibition, and reproductive/developmental biology, investigating IRGC1's importance for fibrous sheath integrity and sperm motility in mice. Both studies utilized the antibody for Western blot analysis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Front Cardiovasc Med; Secreted Frizzled-Related Protein 5 Protects Against Cardiac Rupture and Improves Cardiac Function Through Inhibiting Mitochondrial Dysfunction.; Authors - Xin Huang
mouse	WB	Dev Biol; IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.; Authors - Yuki Kaneda