IRS1 Recombinant antibody, PBS Only

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  WB analysis using 82730-10-RR (same clone as 82730-10-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82730-10-RR (IRS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82730-10-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 82730-10-RR (same clone as 82730-10-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IRS1 antibody (82730-10-RR, Clone: 250510A12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82730-10-PBS in a different storage buffer formulation.

IRS1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82730-10-PBS

Clone No.250510A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HIRS 1, insulin receptor substrate 1, IRS 1, IRS-1

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Product Information

82730-10-PBS targets IRS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRS1 fusion protein Ag11714
• Full Name: insulin receptor substrate 1
• Calculated Molecular Weight: 1242 aa, 132 kDa
• Observed Molecular Weight: 160-185 kDa
• GenBank Accession Number: BC053895
• Gene Symbol: IRS1
• Gene ID (NCBI): 3667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35568
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.