ISM2 Polyclonal antibody

ISM2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21540-1-AP

Synonyms

ISM2, Isthmin 2, isthmin 2 homolog (zebrafish), TAIL1, THSD3



Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse pancreas tissue, mouse skin tissue
Positive IHC detected inhuman placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21540-1-AP is ISM2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISM2 fusion protein Ag16187
Full Name isthmin 2 homolog (zebrafish)
Calculated molecular weight 571 aa, 64 kDa
Observed molecular weight 65 kDa, 55 kDa
GenBank accession numberBC101020
Gene symbol ISM2
Gene ID (NCBI) 145501
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ISM2 antibody 21540-1-APDownload protocol
IHC protocol for ISM2 antibody 21540-1-APDownload protocol
IF protocol for ISM2 antibody 21540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols