ISOC1 Polyclonal antibody

ISOC1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25844-1-AP

Synonyms

CGI 111, ISOC1

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Validation Data Gallery

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  WB analysis using 25844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25844-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 25844-1-AP

  IP result of anti-ISOC1 (IP:25844-1-AP, 4ug; Detection:25844-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse liver tissue, HepG2 cells, SH-SY5Y cells, mouse embryo tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25844-1-AP is ISOC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISOC1 fusion protein Ag22611
• Full Name: isochorismatase domain containing 1
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014105
• Gene Symbol: ISOC1
• Gene ID (NCBI): 51015
• RRID: AB_2918095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.