Tested Applications
| Positive WB detected in | HEK-293 cells, U-251 cells, NIH/3T3 cells, K-562 cells, Hela cells, Raji cells |
| Positive IP detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20920-1-AP targets ITCH in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15120 Product name: Recombinant human ITCH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 556-903 aa of BC011571 Sequence: TLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE Predict reactive species |
| Full Name | itchy E3 ubiquitin protein ligase homolog (mouse) |
| Calculated Molecular Weight | 903 aa, 103 kDa |
| Observed Molecular Weight | 103 kDa, 70 kDa |
| GenBank Accession Number | BC011571 |
| Gene Symbol | ITCH |
| Gene ID (NCBI) | 83737 |
| RRID | AB_2878765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96J02 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITCH is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. It is involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. ITCH can be cleaved by caspase-3, -6, and -7 on residue Asp240 and form a 65 kDa cleaved form (PMID: 19541840).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ITCH antibody 20920-1-AP | Download protocol |
| IP protocol for ITCH antibody 20920-1-AP | Download protocol |
| WB protocol for ITCH antibody 20920-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
20920-1-AP is an anti-ITCH antibody with 27 citations. Publications appear in journals including Nature Cell Biology, Nature Communications, Nature Chemical Biology, Theranostics, Advanced Science, Science Advances, Cancer Letters, Cell Death Discovery, Developmental Cell, Oncogene, Molecular Medicine, Clinical and Translational Medicine, Hepatology International, Cell Reports, Biochemical Pharmacology, International Immunopharmacology, Journal of Nutritional Biochemistry, Journal of Molecular Medicine, FASEB Journal, European Journal of Immunology, Archives of Biochemistry and Biophysics, Frontiers in Oncology, Pediatric Research, Hereditas, Biological and Pharmaceutical Bulletin, and Biology Direct. Research fields span cancer biology, neurodegeneration, fibrosis, immunology, and ubiquitin-mediated protein degradation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome.
| ||
Nat Commun Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets. | ||
Nat Chem Biol µMap proximity labeling in living cells reveals stress granule disassembly mechanisms
| ||
Theranostics ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. | ||
Adv Sci (Weinh) The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer Cells. | ||
Sci Adv ITCH regulates Golgi integrity and proteotoxicity in neurodegeneration.
|
Reviews
What customers say
The single review for this antibody rates it 5 stars. The user tested it on western blot and reported that it successfully detected endogenous ITCH in both SH-SY5Y and HEK293T cell lines. A dilution of 1:1000 was used. Overall, the feedback is positive, confirming the antibody's effectiveness for detecting endogenous ITCH protein in these cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chrisovalantou (Verified Customer) (10-31-2024) | Antibody was able to detech endogenous ITCH in SH-SY5Y and HEK293T on western blot
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