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  • KD/KO Validated

ITCH Polyclonal antibody

ITCH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20920-1-AP

Synonyms

AIF4, AIP4, ITCH, NAPP1, NFE2 associated polypeptide 1



Tested Applications

Positive WB detected inHEK-293 cells, U-251 cells, NIH/3T3 cells, K-562 cells, Hela cells, Raji cells
Positive IP detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20920-1-AP targets ITCH in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITCH fusion protein Ag15120
Full Name itchy E3 ubiquitin protein ligase homolog (mouse)
Calculated Molecular Weight 903 aa, 103 kDa
Observed Molecular Weight 103 kDa, 70 kDa
GenBank Accession NumberBC011571
Gene Symbol ITCH
Gene ID (NCBI) 83737
RRIDAB_2878765
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITCH is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. It is involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. ITCH can be cleaved by caspase-3, -6, and -7 on residue Asp240 and form a 65 kDa cleaved form (PMID: 19541840).

Protocols

Product Specific Protocols
WB protocol for ITCH antibody 20920-1-APDownload protocol
IHC protocol for ITCH antibody 20920-1-APDownload protocol
IP protocol for ITCH antibody 20920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KD Validated
humanWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
  • KD Validated
humanWB

Theranostics

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

Authors - Lijie Zhou
humanWB,IP

Clin Transl Med

GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.

Authors - Qian Yun Ge
  • KD Validated
humanWB, CoIP

Front Oncol

Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.

Authors - Cong Wang
  • KD Validated
humanWB

J Mol Med (Berl)

Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.

Authors - Yuhong Zhang