ITCH Monoclonal antibody

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  WB analysis using 67757-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67757-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67757-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67757-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67757-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67757-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ITCH Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67757-1-Ig

Clone No.2E3G9

Publications(3)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, K-562 cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

67757-1-Ig targets ITCH in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14688

  Product name: Recombinant human ITCH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 556-903 aa of BC011571

  Sequence: TLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

• Full Name: itchy E3 ubiquitin protein ligase homolog (mouse)
• Calculated Molecular Weight: 903 aa, 103 kDa
• Observed Molecular Weight: 103 kDa, 70 kDa
• GenBank Accession Number: BC011571
• Gene Symbol: ITCH
• Gene ID (NCBI): 83737
• RRID: AB_2918525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96J02
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	EMBO J; A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease; Authors - Kyungtae Lim
human	WB,IP	Biomedicines; ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis; Authors - Liansuo Zhang
human	IF	Sci Rep; Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma; Authors - Zehua Lin