Integrin Alpha 3 Polyclonal antibody

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• Integrin Alpha 3 Polyclonal antibody (21992-1-AP)

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Integrin Alpha 3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21992-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, IB

Synonyms

ITGA3, CD49 antigen-like family member C, CD49C, FRP 2, FRP-2

• 

  WB analysis of A549 using 21992-1-AP

  WB result of Integrin Alpha 3 antibody (21992-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21992-1-AP

  A172 cells lysates were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 21992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 21992-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of PC-3 using 21992-1-AP

  IP result of anti-Integrin Alpha 3 (IP:21992-1-AP, 4ug; Detection:21992-1-AP 1:500) with PC-3 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 21992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Integrin Alpha 3 antibody (21992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, A172 cells, human heart tissue, human testis tissue, Calu-1 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21992-1-AP targets Integrin Alpha 3 in WB, IHC, IF/ICC, IP, CoIP, ELISA, IB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17293

  Product name: Recombinant human Integrin alpha-3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 52-376 aa of BC150190

  Sequence: FGYSVALHRQTERQQRYLLLAGAPRELAVPDGYTNRTGAVYLCPLTAHKDDCERMNITVKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAHRYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDDWQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNTVYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDPEDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHRHMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAIYVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI

• Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Calculated Molecular Weight: 1066 aa, 119 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC150190
• Gene Symbol: Integrin alpha 3
• Gene ID (NCBI): 3675
• RRID: AB_10953527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P26006
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin Alpha 3 (ITGA3), also named as CD49c, GAPB3, and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cell migration. ITGA3 is highly concentrated in epithelial cells, where it strongly adheres to Laminin-5, and Laminin-5-induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publications

What published studies show

The anti-Integrin α3 antibody (Cat# 21992-1-AP) has 19 citations published in journals including Nature Communications, Advanced Science, Small, Journal of Advanced Research, Carbohydrate Polymers, Materials Today Bio, Apoptosis, Cancer Gene Therapy, Frontiers in Microbiology, Scientific Reports, Laboratory Investigation, and others. Research fields span cancer biology (glioblastoma, pancreatic, breast, cervical, ovarian, and esophageal cancers), angiogenesis, wound healing, pulmonary fibrosis, integrin signaling, cell migration, and infection.

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF	Nat Commun; Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.; Authors - Eunnyung Bae
human	WB	J Adv Res; OTUD1 delays wound healing by regulating endothelial function and angiogenesis in diabetic mice; Authors - Jiajia Zhang
mouse	WB,IP,IHC,IF,CoIP	Adv Sci (Weinh); Nephronectin (NPNT) is a Crucial Determinant of Idiopathic Pulmonary Fibrosis: Modulating Cellular Senescence via the ITGA3/YAP1 Signaling Axis; Authors - Jiayu Guo ; KD Validated
human	IB	Adv Sci (Weinh); Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53.; Authors - Roberta Cacciatore
mouse	IF	Carbohydr Polym; High molecular weight laminarin/AgNPs-impregnated PVA based in situ hydrogels accelerated diabetic wound healing.; Authors - Yasir Faraz Abbasi
human		Small; Mimicking Tumor Metastasis Using a Transwell-Integrated Organoids-On-a-Chip Platform; Authors - Maike Chen