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CD11B/Integrin Alpha M Polyclonal antibody

CD11B/Integrin Alpha M Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21851-1-AP

Synonyms

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6



Tested Applications

Positive WB detected inTF-1 cells
Positive IHC detected inhuman tonsillitis tissue, Insulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21851-1-AP targets CD11B/Integrin Alpha M in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11B/Integrin Alpha M fusion protein Ag16327
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 1152 aa, 127 kDa
Observed molecular weight170 kDa
GenBank accession numberBC096346
Gene symbol ITGAM
Gene ID (NCBI) 3684
RRIDAB_2878927
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
WB protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
IHC protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
IF protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Aging Cell

Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.

Authors - Zhi Chen
FC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
IF

ACS Appl Mater Interfaces

Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets

Authors - Lirong Yi
IF

Int J Biol Macromol

Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages

Authors - Renhao Ni
IF

Biomater Sci

A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring

Authors - Ximeng Kang
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan