CD11B/Integrin Alpha M Polyclonal antibody

CD11B/Integrin Alpha M Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 21851-1-AP

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, Insulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21851-1-AP is CD11B/Integrin Alpha M Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11B/Integrin Alpha M fusion protein Ag16327
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated molecular weight 1152 aa, 127 kDa
GenBank accession numberBC096346
Gene symbol ITGAM
Gene ID (NCBI) 3684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
IHC protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Oncol

High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas.

Authors - Weilun Fu
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan
humanIF,IHC

Aging (Albany NY)

Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma.

Authors - Junfeng Zhang
mouseWB

Mol Pain

Short-chain fatty acids contribute to neuropathic pain via regulating microglia activation and polarization.

Authors - Feng Zhou
mouseWB,IHC

Cytokine

IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.

Authors - Zhihao Wang
mouseFC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang