Integrin Alpha V Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Alpha V Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 27096-1-AP

Synonyms

CD51, Integrin alpha V, ITGAV, MSK8, VNRA

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Validation Data Gallery

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  WB analysis using 27096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27096-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, A549 cells, MCF-7 cells, human placenta tissue
• Positive IHC detected in: mouse kidney tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

27096-1-AP targets Integrin Alpha V in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Full Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Calculated molecular weight: 1048 aa, 116 kDa
• Observed molecular weight: 130 kDa
• GenBank accession number: BC136442
• Gene symbol: ITGAV
• Gene ID (NCBI): 3685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Aging Cell; Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.; Authors - Can Hu ; KD Validated
human	WB	Curr Biol; The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.; Authors - Adrien Presle
human	WB,IHC	Int J Mol Sci; Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression.; Authors - Justin K Messex
human	WB	Int J Mol Sci; Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.; Authors - Emma Plana
human	IHC	Cancer Cell Int; Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.; Authors - Zijian Da
human	WB	J Exp Clin Cancer Res; Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.; Authors - Feikai Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF