Integrin Alpha V Polyclonal antibody

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  WB analysis using 27096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27096-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27096-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 27096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 27096-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

Integrin Alpha V Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27096-1-AP

Publications(20)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ITGAV, CD51, Integrin alpha-V, Integrin alpha-V heavy chain, Integrin alpha-V light chain

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Tested Applications

• Positive WB detected in: HUVEC cells, A549 cells, MCF-7 cells, human placenta tissue
• Positive IHC detected in: mouse kidney tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

27096-1-AP targets Integrin Alpha V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha V fusion protein Ag25825
• Full Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Calculated Molecular Weight: 1048 aa, 116 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136442
• Gene Symbol: Integrin alpha V
• Gene ID (NCBI): 3685
• RRID: AB_2880753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06756
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2; Authors - Zirong Chen
rat	WB	Aging Cell; Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.; Authors - Can Hu ; KD Validated
human	WB	Curr Biol; The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.; Authors - Adrien Presle
human	WB,IHC	Int J Mol Sci; Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression.; Authors - Justin K Messex
human	WB	Cell Signal; C1GALT1 high expression enhances the progression of glioblastoma through the EGFR-AKT/ERK cascade; Authors - Yanting Su
human	WB	Int J Mol Sci; Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.; Authors - Emma Plana

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alex (Verified Customer) (03-31-2025)

Exceptional focal adhesion staining in LN229 cells. Identity of the nuclear stain is unknown. Grayscale = Itgav antibody at 1:200. 4% formaldehyde fixation (methanol-free) with 0.1% Triton X-100 permeabilisation. Blocked in 2% BSA / 0.1% Triton X-100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Neural - LN229

Integrin Alpha V Antibody Immunofluorescence validation (1:200 dilution) in Neural - LN229 (Cat no:27096-1-AP)

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF