• Featured Product
  • KD/KO Validated

Integrin Alpha V Polyclonal antibody

Integrin Alpha V Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 27096-1-AP


CD51, Integrin alpha V, ITGAV, MSK8, VNRA

Tested Applications

Positive WB detected inHUVEC cells, A549 cells, MCF-7 cells, human placenta tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27096-1-AP targets Integrin Alpha V in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha V fusion protein Ag25825
Full Name integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Calculated Molecular Weight 1048 aa, 116 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC136442
Gene Symbol ITGAV
Gene ID (NCBI) 3685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for Integrin Alpha V antibody 27096-1-APDownload protocol
IHC protocol for Integrin Alpha V antibody 27096-1-APDownload protocol
IF protocol for Integrin Alpha V antibody 27096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF