ITIH4 Polyclonal antibody

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  WB analysis of human plasma using 24069-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24069-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

ITIH4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24069-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Inter-alpha-trypsin inhibitor heavy chain H4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, Inter-alpha-inhibitor heavy chain 4, IHRP, 70 kDa inter-alpha-trypsin inhibitor heavy chain H4

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Tested Applications

• Positive WB detected in: human plasma, mouse lung tissue, mouse serum, rat serum
• Positive IHC detected in: human hepatocirrhosis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

24069-1-AP targets ITIH4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITIH4 fusion protein Ag21275
• Full Name: inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136393
• Gene Symbol: ITIH4
• Gene ID (NCBI): 3700
• RRID: AB_2879426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q14624
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cardiovasc Res; Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis; Authors - Aarthi Ravindran
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IHC	Front Oncol; Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.; Authors - Changhong Yang
mouse	WB	Clin Exp Immunol; Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.; Authors - Atsumu Osada
	WB,IHC	BMC Cancer; Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.; Authors - Naohiko Nakamura
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang