ITIH4 Polyclonal antibody

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  WB analysis of human plasma using 24069-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24069-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 24069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

ITIH4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24069-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

35 kDa inter-alpha-trypsin inhibitor heavy chain H4, 70 kDa inter-alpha-trypsin inhibitor heavy chain H4, IHRP, Inter-alpha-inhibitor heavy chain 4, Inter-alpha-trypsin inhibitor family heavy chain-related protein

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Tested Applications

• Positive WB detected in: human plasma, mouse lung tissue, mouse serum, rat serum
• Positive IHC detected in: human hepatocirrhosis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

24069-1-AP targets ITIH4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITIH4 fusion protein Ag21275
• Full Name: inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136393
• Gene Symbol: ITIH4
• Gene ID (NCBI): 3700
• RRID: AB_2879426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q14624
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITIH4(Inter-alpha-trypsin inhibitor heavy chain H4) is a liver-restricted plasma glycoprotein belonging to the serine protease inhibitor family, playing diverse roles in inflammation, extracellular matrix (ECM) stabilization, and cancer progression. Expressed mainly in hepatocytes, ITIH4 is secreted into the bloodstream, where it can be cleaved by plasma kallikrein into specific fragments. Functionally, ITIH4 acts as an anti-apoptotic and matrix-stabilizing molecule during liver development and regeneration, with its expression regulated by interleukin-6 (IL-6).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cardiovasc Res; Translatome profiling reveals Itih4 as a novel smooth muscle cell-specific gene in atherosclerosis; Authors - Aarthi Ravindran
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IHC	Front Oncol; Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.; Authors - Changhong Yang
mouse	WB	Clin Exp Immunol; Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.; Authors - Atsumu Osada
	WB,IHC	BMC Cancer; Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.; Authors - Naohiko Nakamura
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang