Tested Applications
| Positive WB detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14407-1-AP targets ITM2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5692 Product name: Recombinant human ITM2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC040437 Sequence: MVKIAFNTPTAVQKEEARQDVEALLSRTVRTQILTGKELRVATQEKEGSSGRCMLTLLGLSFILAGLIVGGACIYKYFMPKSTIYRGEMCFFDSEDPANSLRGGEPNFLPVTEEADIREDDNIAIIDVPVPSFSDSDPAAIIHDFEKGMTAYLDLLLGNCYLMPLNTSIVMPPKNLVELFGKLASGRYLPQTYVVREDLVAVEEIRDVSNLGIFIYQLCNNRKSFRLRRRDLLLGFNKRAIDKCWKIRHFPNEFIVETKICQE Predict reactive species |
| Full Name | integral membrane protein 2A |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC040437 |
| Gene Symbol | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2129683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integral membrane protein 2A (ITM2A) is a single-pass type II membrane protein belonging to a family,which is composed of at least two other members ITM2B and ITM2C. ITM2A can be induced during thymocyte selection and T cell activation. In early stages of differentiation ITM2A may be associated with an inhibition of the initiation of chondrogenesis.Catalog#14407-1-AP recognises two band of 30 kDa and 45-48 kDa, and the additional 45-48 kDa band may be due to glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 14407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ITM2A Polyclonal antibody (Cat# 14407-1-AP) has 4 citations published in Frontiers in Immunology, Frontiers in Pharmacology, Frontiers in Oncology, and Adipocyte. Research fields include head and neck squamous carcinoma immune microenvironment, monocyte heterogeneity in sepsis, breast cancer tumor suppression and PD-L1 correlation, and adipocyte differentiation regulation.
| Species | Application | Title |
|---|---|---|
Front Immunol Integrating machine learning, bioinformatics and experimental verification to identify a novel prognostic marker associated with tumor immune microenvironment in head and neck squamous carcinoma | ||
Front Pharmacol Decoding monocyte heterogeneity in sepsis: a single-cell apoptotic signature for immune stratification and guiding precision therapy. | ||
Adipocyte Itm2a silencing rescues lamin A mediated inhibition of 3T3-L1 adipocyte differentiation.
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