ITM2A Polyclonal antibody

• 

  WB analysis of mouse lung using 14407-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14407-1-AP (ITM2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

ITM2A Polyclonal Antibody for WB, ELISA

Cat No. 14407-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BRICD2A, E25A, integral membrane protein 2A, ITM2A, Protein E25

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Tested Applications

• Positive WB detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

14407-1-AP targets ITM2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5692

  Product name: Recombinant human ITM2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-263 aa of BC040437

  Sequence: MVKIAFNTPTAVQKEEARQDVEALLSRTVRTQILTGKELRVATQEKEGSSGRCMLTLLGLSFILAGLIVGGACIYKYFMPKSTIYRGEMCFFDSEDPANSLRGGEPNFLPVTEEADIREDDNIAIIDVPVPSFSDSDPAAIIHDFEKGMTAYLDLLLGNCYLMPLNTSIVMPPKNLVELFGKLASGRYLPQTYVVREDLVAVEEIRDVSNLGIFIYQLCNNRKSFRLRRRDLLLGFNKRAIDKCWKIRHFPNEFIVETKICQE

• Full Name: integral membrane protein 2A
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC040437
• Gene Symbol: ITM2A
• Gene ID (NCBI): 9452
• RRID: AB_2129683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integral membrane protein 2A (ITM2A) is a single-pass type II membrane protein belonging to a family,which is composed of at least two other members ITM2B and ITM2C. ITM2A can be induced during thymocyte selection and T cell activation. In early stages of differentiation ITM2A may be associated with an inhibition of the initiation of chondrogenesis.Catalog#14407-1-AP recognises two band of 30 kDa and 45-48 kDa, and the additional 45-48 kDa band may be due to glycosylation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Front Oncol; ITM2A as a Tumor Suppressor and Its Correlation With PD-L1 in Breast Cancer.; Authors - Rui Zhang
mouse	WB	Adipocyte; Itm2a silencing rescues lamin A mediated inhibition of 3T3-L1 adipocyte differentiation.; Authors - Stephanie J Davies ; KD Validated
human	WB,IHC	Front Immunol; Integrating machine learning, bioinformatics and experimental verification to identify a novel prognostic marker associated with tumor immune microenvironment in head and neck squamous carcinoma; Authors - Xiaoxia Zeng