Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18306-1-AP targets ITM2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | integral membrane protein 2A |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC040437 |
| Gene Symbol | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2249412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITM2A, also named BRICD2A and E25A, is a member of type II integral membrane (ITM2) protein family. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. There are 5 articles specifically referring to this gene in PubMed. Proteins are expected to localize in various compartments (cytoplasm, integral to membrane). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 18306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ITM2A Polyclonal antibody (Cat# 18306-1-AP) has 9 citations published in Blood, Bone Research, Cell Communication and Signaling, Fluids and Barriers of the CNS, Nutrients, iScience, Archives of Biochemistry and Biophysics, PLoS ONE, and COPD. Associated research fields include Th17 cell differentiation, angiogenesis and heterotopic ossification, breast cancer autophagy, brain drug delivery, colorectal cancer, skeletal muscle regeneration, hepatocellular carcinoma ferroptosis, dental development, and COPD progression.
| Species | Application | Title |
|---|---|---|
Blood Identification of early gene expression changes during human Th17 cell differentiation. | ||
Bone Res Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice. | ||
Cell Commun Signal Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. | ||
Nutrients Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment | ||
iScience The regenerating skeletal muscle niche drives satellite cell return to quiescence. |
