ITPKB Polyclonal antibody

• Featured Product
• KD/KO Validated

ITPKB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12816-1-AP

Synonyms

InsP 3 kinase B, IP3 3 kinase B, IP3K, IP3K B, IP3KB, ITPKB, PIG37

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Validation Data Gallery

• 

  WB analysis of mouse brain using 12816-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12816-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12816-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12816-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12816-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12816-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12816-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12816-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12816-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12816-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12816-1-AP (ITPKB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, K-562 cells, mouse lung tissue, mouse thymus tissue, rat brain tissue
• Positive IHC detected in: human cervical cancer tissue, human lung tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

12816-1-AP targets ITPKB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITPKB fusion protein Ag3456
• Full Name: inositol 1,4,5-trisphosphate 3-kinase B
• Calculated Molecular Weight: 946 aa, 102 kDa
• Observed Molecular Weight: 102-110 kDa
• GenBank Accession Number: BC015009
• Gene Symbol: ITPKB
• Gene ID (NCBI): 3707
• RRID: AB_2129846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)

Publications

Species	Application	Title
human	WB,IHC	Signal Transduct Target Ther; Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis; Authors - Yuanliang Yan ; KD Validated
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
human	WB	J Allergy Clin Immunol; Severe combined immunodeficiency caused by inositol-trisphosphate 3-kinase B (ITPKB) deficiency.; Authors - Abduarahman Almutairi
human	WB,IF	EMBO Mol Med; miR-132 loss de-represses ITPKB and aggravates amyloid and TAU pathology in Alzheimer's brain.; Authors - Evgenia Salta ; KD Validated
mouse	IHC, WB	Sci Signal; Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca2+ mobilization and the inflammatory activity of dendritic cells.; Authors - Laura Marongiu
human	WB,IF	Int J Mol Sci; Increased Levels of the Parkinson's Disease-Associated Gene ITPKB Correlate with Higher Expression Levels of α-Synuclein, Independent of Mutation Status; Authors - Francesca Di Leva