Intersectin 1 Polyclonal antibody

Intersectin 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21862-1-AP

Synonyms

ITSN1, SH3 domain-containing protein 1A, ITSN 1, ITSN, Intersectin-1

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Validation Data Gallery

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  WB analysis of fetal human brain using 21862-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21862-1-AP (Intersectin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: human stomach tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21862-1-AP targets Intersectin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Intersectin 1 fusion protein Ag16517
• Full Name: intersectin 1 (SH3 domain protein)
• Calculated Molecular Weight: 1721 aa, 195 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC116186
• Gene Symbol: Intersectin 1
• Gene ID (NCBI): 6453
• RRID: AB_2878931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intersectin 1 (ITSN1) is a multidomain adaptor protein that plays a crucial role in various cellular processes, particularly in the nervous system. It is involved in clathrin-mediated endocytosis, signal transduction, and the regulation of the actin cytoskeleton. The protein is highly abundant in neurons and has been implicated in neurodevelopmental disorders, such as autism spectrum disorders, intellectual disability, and epilepsy. Recent studies have identified de novo variants in the ITSN1 gene in patients with these disorders, suggesting a link between ITSN1 and brain development.

Publications

Species	Application	Title
human	WB	Front Oncol; Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions.; Authors - Rui Wei