Tested Applications
| Positive WB detected in | MCF-7 cells, human brain tissue, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10822-1-AP targets IVD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat, arabidopsis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1252 Product name: Recombinant human IVD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-423 aa of BC017202 Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH Predict reactive species |
| Full Name | isovaleryl Coenzyme A dehydrogenase |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 41-46 kDa |
| GenBank Accession Number | BC017202 |
| Gene Symbol | IVD |
| Gene ID (NCBI) | 3712 |
| RRID | AB_2296258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26440 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IVD antibody 10822-1-AP | Download protocol |
| IHC protocol for IVD antibody 10822-1-AP | Download protocol |
| IP protocol for IVD antibody 10822-1-AP | Download protocol |
| WB protocol for IVD antibody 10822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IVD (Isovaleryl CoA dehydrogenase, mitochondrial) polyclonal antibody (Cat# 10822-1-AP) has 8 citations spanning journals including J Transl Med, Cell Mol Life Sci, Plant J, Plant Cell Rep, J Neurochem, FASEB J, Mol Endocrinol, and Proteomics Clin Appl. Research fields cover diabetic cardiomyopathy, mitochondrial one-carbon metabolism, plant amino acid catabolism, neuronal seizure mechanisms, ferroptosis in cardiac hypertrophy, islet beta-cell proteomics, and synaptic mitochondrial bioenergetics.
| Species | Application | Title |
|---|---|---|
J Transl Med Role of mitochondrial metabolic disorder and immune infiltration in diabetic cardiomyopathy: new insights from bioinformatics analysis | ||
Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. | ||
Plant Cell Rep The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures. | ||
J Neurochem Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure. | ||
FASEB J Modulation of Ferroptosis by the Irx3-Etfa Pathway Protects Against Cardiac Hypertrophy. |
Reviews
What customers say
The single review reports a 5-star rating for Western Blot. The antibody was used at a 1:1000 dilution on mitochondrial fractions of a mouse striatal cell line, with overnight primary incubation at 4°C and an IRDye 680LT goat anti-rabbit secondary. The reviewer provided a clear WB image alongside SDS-PAGE and transfer details, confirming specific band detection. Overall, the feedback indicates reliable performance and good signal in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lana (Verified Customer) (06-21-2019) | Lines on WB:1 - BioRad Precision Plus Protein standards2 and 3 – biological replicates, lysates of mitochondrial fractions of ST-Hdh-Q7/Q7 mouse striatal cell line.SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.
 |
