ICAM-1/CD54 Polyclonal antibody

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• ICAM-1/CD54 Polyclonal antibody (30324-1-AP)

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 30324-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Icam-1, CD54, MALA-2, Icam1, ICAM 1

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  WB analysis using 30324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30324-1-AP (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 30324-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 30324-1-AP (Icam-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse liver using 30324-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue
• Positive IHC detected in: mouse liver tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30324-1-AP targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_3669710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1.

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this antibody is highly positive, rated 5 out of 5 stars. The reviewer used it at a 1:100 dilution for immunofluorescence on murine inflamed colon tissue and reported that it worked perfectly in their stainings. Overall, the feedback indicates strong performance and reliability for the application tested.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Julia (Verified Customer) (05-07-2026)

This antibody works perfect in my stainings!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: murin inlfamed colon tissue