CLNS1A/CLCI Polyclonal antibody

CLNS1A/CLCI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25964-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CLNS1A, ICln, Chloride conductance regulatory protein ICln (I(Cln)), CLCI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 25964-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (CLNS1A/CLCI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MDCK using 25964-1-AP
MDCK cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (CLNS1A/CLCI antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25964-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 25964-1-AP (CLNS1A/CLCI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 25964-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25964-1-AP (CLNS1A/CLCI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HEK-293 cells, MDCK cells
Positive IHC detected in	human colon cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

25964-1-AP targets CLNS1A/CLCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22923 Product name: Recombinant human CLNS1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC119634 Sequence: MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPEDEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH Predict reactive species
Full Name	chloride channel, nucleotide-sensitive, 1A
Calculated Molecular Weight	237 aa, 26 kDa
Observed Molecular Weight	36-42 kDa
GenBank Accession Number	BC119634
Gene Symbol	CLNS1A
Gene ID (NCBI)	1207
RRID	AB_2880312
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54105
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for CLNS1A/CLCI antibody 25964-1-AP	Download protocol
IHC protocol for CLNS1A/CLCI antibody 25964-1-AP	Download protocol
WB protocol for CLNS1A/CLCI antibody 25964-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
	WB	BMC Genomics Mechanisms underlying the estradiol mediated protection of yak oviduct epithelial cells against ER-Ca2+ imbalance Authors - Xiaolin Ye View Article