Icln Polyclonal antibody

Icln Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25964-1-AP

Synonyms

CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln

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Validation Data Gallery

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  WB analysis of HEK-293 using 25964-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 25964-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 25964-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25964-1-AP (Icln antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MDCK cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25964-1-AP is Icln Fusion Protein expressed in E. coli.

• Tested Reactivity: human, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Icln fusion protein Ag22923
• Full Name: chloride channel, nucleotide-sensitive, 1A
• Calculated Molecular Weight: 237 aa, 26 kDa
• Observed Molecular Weight: 36-42 kDa
• GenBank Accession Number: BC119634
• Gene Symbol: CLNS1A
• Gene ID (NCBI): 1207
• RRID: AB_2880312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.