Rabbit IgG control Polyclonal antibody
Rabbit IgG control Polyclonal Antibody for FC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Applications
WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA
Conjugate
Unconjugated
250
Cat no : 30000-0-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Protein, Control |
Positive IP detected in | Control |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30000-0-AP targets Rabbit IgG control in WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with samples.
Tested Reactivity | |
Cited Reactivity | human, mouse, rat, sheep, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | N/A |
Full Name | Rabbit IgG control |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2819035 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
Species | Application | Title |
---|---|---|
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis | ||
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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FH Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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FH Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
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FH Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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FH Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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FH Priyanka (Verified Customer) (12-18-2018) |
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FH Seongjin (Verified Customer) (12-14-2018) |
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