Tested Applications
| Positive WB detected in | Control |
| Positive IP detected in | Control |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, IP-MS, ELISA, Blocking assay, Cell treatment, CUT&RUN applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Cited Reactivity | hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | Rabbit IgG control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
What published studies show
The Rabbit IgG control antibody (Cat# 30000-0-AP) has accumulated 1,159 citations across 45+ journals including Nature, Cancer Cell, Cell, Cell Metabolism, Nature Communications, Autophagy, Molecular Cell, and Immunity. Research fields span oncology (cancer progression, immune evasion, drug resistance), immunology, metabolism (ferroptosis, lipid metabolism), autophagy, epigenetics, virology, cardiology, and neuroscience. Cited applications include IP, CoIP, ChIP, RIP, WB, IHC, and ELISA, reflecting its broad utility as an isotype control in diverse experimental systems.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy | ||
Signal Transduct Target Ther Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification | ||
Cancer Cell Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8(+) T cell cytotoxicity and therapeutic response. | ||
Cancer Cell Interferon-dependent SLC14A1(+) cancer-associated fibroblasts promote cancer stemness via WNT5A in bladder cancer | ||
Reviews
What customers say
Users consistently rate this product 4–5 stars across 10 reviews. It is most frequently praised as an effective negative control for immunoprecipitation, with clean results in Jurkat, glioblastoma, and C. elegans samples. Several reviewers highlight its efficiency in Western Blot at 1:10,000 dilution and its utility in neutralization experiments. One reviewer noted the antibody is highly concentrated and recommended using less than the typical amount. Overall, customers describe it as reliable, well-performing, and a solid isotype control for IP, WB, and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (11-20-2025) |
|
Roy (Verified Customer) (10-07-2025) | Perfect control for IP
|
Celine (Verified Customer) (07-09-2025) | Works well as a negative control for immunoprecipitation
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Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
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Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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Priyanka (Verified Customer) (12-18-2018) |
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Seongjin (Verified Customer) (12-14-2018) |
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