Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

and More (5)

Applications

WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 30000-0-AP

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin, RIGGCON



Tested Applications

Positive WB detected inProtein, Control
Positive IP detected inControl
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

30000-0-AP targets Rabbit IgG control in WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with samples.

Tested Reactivity
Cited Reactivityhuman, mouse, rat, sheep, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A
Full Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
RRIDAB_2819035
Conjugate Unconjugated
Form Liquid
Purification Method Protein A affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

SpeciesApplicationTitle
IP

Drug Resist Updat

CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis

Authors - Jing Wu
human,mouseWB

Cell Res

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Authors - Ying Gao
IP

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
IP

ACS Nano

Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling

Authors - Bide Tong
WB

Mol Cell

CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity

Authors - Guiheng Zhang
IP

Mol Cell

Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy.

Authors - Lulu Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Siddharth (Verified Customer) (08-02-2023)

Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.

  • Applications: Western Blot
FH

Macarena (Verified Customer) (10-07-2022)

very concentrated. Excellent results. Deffinetly use less than you would normally use.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: cell lines
FH

Guo-rong (Verified Customer) (08-30-2022)

It works well as an antibody control in neutralization experiments

  • Applications: Cell culture
  • Cell Tissue Type: ARPE-19
FH

Anita (Verified Customer) (01-04-2022)

Works well as a control for IP

  • Applications: Immunoprecipitation
  • Cell Tissue Type: C. elegans
FH

Kenneth (Verified Customer) (02-14-2019)

Great company with Great products. Highly recommended.

  • Applications: Western Blot,
FH

Priyanka (Verified Customer) (12-18-2018)

  • Applications: Western Blot, Immunofluorescence,
FH

Seongjin (Verified Customer) (12-14-2018)

  • Applications: Immunoprecipitation,