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Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
65208-1-Ig targets IgG2a Isotype Control in FC, IP, CoIP applications and shows reactivity with samples.
| Tested Reactivity | |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG2a Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918470 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2a Isotype Control antibody 65208-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification | ||
J Nutr Sci Vitaminol (Tokyo) Cooperation of Cysteine, Ascorbic Acid, Pyridoxine, and α-Lipoic Acid in Antioxidant Effects on Human Keratinocytes and 3D Human Skin Models | ||
Mol Med Progesterone (P4) ameliorates cigarette smoke-induced chronic obstructive pulmonary disease (COPD) |
