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1x10^6 human PBMCs were stained with 0.25 ug CoraLux Violet 510 Mouse IgG2a Isotype Control (MOPC-173) RecAb (CLV510-65571, Clone: MOPC-173) (red), or 0.25 ug CoraLux Violet 510 Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody (CLV510-65603, Clone: OKT4) (green), or unstained (blue). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 human PBMCs were stained with 0.25 ug CoraLux Violet 510 Mouse IgG2a Isotype Control (MOPC-173) RecAb (CLV510-65571, Clone: MOPC-173) (red), or 0.25 ug CoraLux Violet 510 Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody (CLV510-65603, Clone: OKT4) (green), or unstained (blue). Cells were not fixed.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CLV510-65571 targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
An IgG2a isotype control is a type of negative control antibody used primarily in flow cytometry to distinguish specific antibody staining from non-specific background signals
Protocols
Product Specific Protocols
FC protocol for CoraLux™ Violet 510 IgG2a Isotype Control antibody CLV510-65571
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
FC Figures
FC experiment of human PBMCs using CLV510-65571
1x10^6 human PBMCs were stained with 0.25 ug CoraLux Violet 510 Mouse IgG2a Isotype Control (MOPC-173) RecAb (CLV510-65571, Clone: MOPC-173) (red), or 0.25 ug CoraLux Violet 510 Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody (CLV510-65603, Clone: OKT4) (green), or unstained (blue). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2a Isotype Control Recombinant antibody
Catalog Number
CLV510-65571
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
