Phospho-IkB Alpha (Ser32/36) Monoclonal antibody

• Home
• Products
• Primary Antibodies
• Phospho-IkB Alpha (Ser32/36) Monoclonal antibody (68999-1-Ig)

• 

  WB analysis using 68999-1-Ig

  Untreated PC-3 cells, PC-3 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

• 

  WB analysis using 68999-1-Ig

  Untreated MCF-7 cells, MCF-7 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

Phospho-IkB Alpha (Ser32/36) Monoclonal Antibody for WB, ELISA

Cat No. 68999-1-Ig

Clone No.2G8E5

Publications(5)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, ELISA

Synonyms

P IkB alpha, P IκB α, Pho-IkB Alpha, Pho-IkBα, Phospho IkB Alpha

---

Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells, Calyculin A treated MCF-7 cells, Calyculin A treated PC cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

68999-1-Ig targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_3670459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25963
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
monkey	WB	Front Vet Sci; Potential mechanisms of zinc sulfate in inhibiting porcine reproductive and respiratory syndrome virus replication: attenuation of oxidative stress, inflammatory response, and apoptosis.; Authors - Huizhen Yang
		Front Physiol; Aerobic exercise ameliorates atherosclerosis-induced cognitive impairment via hippocampal IL-33/NF-κB signaling modulation; Authors - Jianwei Peng
human	WB	Free Radic Biol Med; lncRNA-driven multi-omics reconstitution engineers EVs for macrophage-targeted and anti-inflammatory therapy in MASLD.; Authors - Lin Liu
mouse	WB	Int Immunopharmacol; Activation of GPR35 by kynurenic acid inhibits IL-1β secretion in macrophages during CR-hvKP-induced pneumonia; Authors - Qingyue Zhang
mouse	WB	Neuromolecular Med; Xanthoxylin Regulating the Inflammatory Microenvironment After Spinal Cord Injury Through Inhibition of the NF-κB Signaling Pathway.; Authors - Zihao Fan