Phospho-IkB Alpha (Ser32/36) Monoclonal antibody

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• Phospho-IkB Alpha (Ser32/36) Monoclonal antibody (68999-1-Ig)

Phospho-IkB Alpha (Ser32/36) Monoclonal Antibody for WB, ELISA

Cat No. 68999-1-Ig Copied!

Clone No. 2G8E5

Publications (12)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, ELISA

Synonyms

P IkB alpha, P IκB α, Pho-IkB Alpha, Pho-IkBα, Phospho IkB Alpha

• 

  WB analysis using 68999-1-Ig

  Untreated PC-3 cells, PC-3 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 68999-1-Ig

  Untreated MCF-7 cells, MCF-7 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells, Calyculin A treated MCF-7 cells, Calyculin A treated PC cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68999-1-Ig targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_3670459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25963
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).

Publications

What published studies show

Phospho-IκBα (Ser32/36) Monoclonal antibody (Cat# 68999-1-Ig) has 12 citations published in journals including Advanced Science, Food Research International, International Journal of Biological Macromolecules, Free Radical Biology and Medicine, European Journal of Medicinal Chemistry, Ecotoxicology and Environmental Safety, International Immunopharmacology, Frontiers in Physiology, Neuromolecular Medicine, and Frontiers in Veterinary Science. Research fields span NF-κB signaling, inflammation, glioblastoma, metabolic disorders, acute lung injury, stroke, atherosclerosis, spinal cord injury, and viral infection.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); A Computational Strategy for Identifying Self-Assembling Food-Derived Molecules for Antiparasitic Nanotherapy.; Authors - Shenye Qu
mouse	WB	Food Res Int; The nutritional evaluation and hypoglycemic activity of chickpea (Cicer arietinum L.) proteins in vivo based on network pharmacology.; Authors - Ahmidin Wali
		Int J Biol Macromol; A positive feedback loop of HK3-mediated activation of NF-κB modulates glucose metabolism and promotes proliferation and metastasis in glioblastoma.; Authors - Jian Li
human	WB	Free Radic Biol Med; lncRNA-driven multi-omics reconstitution engineers EVs for macrophage-targeted and anti-inflammatory therapy in MASLD.; Authors - Lin Liu
human	WB	Eur J Med Chem; Design, synthesis and biological evaluation of novel pyrrolo[2,3-d]pyrimidine derivatives as cathepsin L inhibitor for the treatment of acute lung injury.; Authors - Zhengtong Mao
		Eur J Med Chem; Design and optimization of N-(3-((4-(1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)amide derivatives as potent anti-inflammatory agents against LPS-induced acute lung injury.; Authors - Zhe Wang