Ins1 Polyclonal antibody

Ins1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28588-1-AP

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I, Insulin-1



Tested Applications

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28588-1-AP targets Ins1 in WB, IHC,ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ins1 fusion protein Ag29988
Full Name insulin I
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2881177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

Protocols

Product Specific Protocols
WB protocol for Ins1 antibody 28588-1-APDownload protocol
IHC protocol for Ins1 antibody 28588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols