Integrin alpha-1 Polyclonal antibody

Integrin alpha-1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 29042-1-AP

Synonyms

CD49a, Integrin alpha 1, integrin, alpha 1, ITGA1, Laminin and collagen receptor, VLA 1, VLA1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, rat lung tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

29042-1-AP targets Integrin alpha-1 in WB, IHC,ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha-1 fusion protein Ag30613
Full Name integrin, alpha 1
Calculated molecular weight 1179 aa, 131 kDa
Observed molecular weight180 kDa
GenBank accession numberBC137121
Gene symbol ITGA1
Gene ID (NCBI) 3672
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-1 antibody 29042-1-APDownload protocol
IHC protocol for Integrin alpha-1 antibody 29042-1-APDownload protocol
Standard Protocols
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