Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody

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• Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR)

Integrin alpha-5/CD49e Uni-rAb^® Recombinant Antibody for FC

Cat No. 98171-1-RR Copied!

Clone No. 241685D4

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Integrin Alpha-5, ITGA5, 241685D4, CD49 antigen-like family member E, CD49e

• 

  FC experiment of human PBMCs using 98171-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98171-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98171-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98171-1-RR against Human Integrin alpha-5/CD49e were performed. The affinity constant is 0.739 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98171-1-RR targets Integrin alpha-5/CD49e in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• RRID: AB_3672314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08648
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Publications

What published studies show

The Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (241685D4), catalog number 98171-1-RR, has 1 citation. It appears in Cell Signaling (2026), in a study investigating RING1 as an Integrin α5 E3 ubiquitin ligase that inhibits esophageal squamous cell carcinoma cell migration and metastasis. The associated research fields include cancer biology, cell adhesion, ubiquitination, and tumor metastasis.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Cell Signal; RING1 is an Integrin α5 E3 ubiquitin ligase and inhibits esophageal squamous cell carcinoma cell migration and metastasis.; Authors - Jingya Guo