Integrin alpha-8 Polyclonal antibody

Integrin alpha-8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55068-1-AP

Synonyms

Integrin alpha 8, Integrin alpha-8, integrin, alpha 8, ITGA8

---

Validation Data Gallery

• 

  WB analysis using 55068-1-AP

  Mouse lung tissue was subjected to SDS PAGE followed by western blot with 55068-1-AP (Integrin alpha-8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55068-1-AP targets Integrin alpha-8 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha 8
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003638
• Gene Symbol: ITGA8
• Gene ID (NCBI): 8516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).