Anti-Human Integrin beta 4 Rabbit Recombinant Antibody

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• Anti-Human Integrin beta 4 Rabbit Recombinant Antibody (98774-1-RR)

Integrin beta 4 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98774-1-RR

Clone No.252740E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

ITGB4, CD104, GP150, Integrin beta-4, integrin, beta 4

• 

  IHC staining of human skin using 98774-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 98774-1-RR (Integrin beta 4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 98774-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 98774-1-RR (Integrin beta 4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 98774-1-RR

  1x10^6 A431 cells were surface stained with 0.25 ug Anti-Human Integrin beta 4 Rabbit RecAb (98774-1-RR, Clone: 252740E5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HT-29 using 98774-1-RR

  1x10^6 HT-29 cells were surface stained with 0.25 ug Anti-Human Integrin beta 4 Rabbit RecAb (98774-1-RR, Clone: 252740E5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 98774-1-RR

  Biolayer interferometry (BLI) kinetic assays of 98774-1-RR against Human Integrin beta 4 were performed. The affinity constant is 4.58 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells, HT-29 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98774-1-RR targets Integrin beta 4 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10479

  Product name: Recombinant human ITB4 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011436 of

  Sequence: \

• Full Name: integrin, beta 4
• Gene Symbol: Integrin beta 4
• Gene ID (NCBI): 3691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.