JAM-A/CD321 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00247-2

  Sandwich ELISA standard curve of MP00247-2, Human JAM-A/Junctional Adhesion Molecule A Recombinant Matched Antibody Pair - PBS only. 84720-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1372. 84720-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

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  Sample test of MP00247-2

  Serum of eight individual healthy human donors was measured. The JAM-A/Junctional Adhesion Molecule A concentration of detected samples was determined to be 2.4 ng/mL with a range of 1.1-5.5 ng/mL.

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  Sample test of MP00247-2

  The mean JAM-A/Junctional Adhesion Molecule A concentration was determined to be 248.8 ng/mL in HUVEC cell extract based on a 1.1 mg/mL extract load and 53.3 ng/mL in U-87 MG cell extract based on a 2.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP00247-2

  Cytometric bead array standard curve of MP00247-2, F11R Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84720-1-PBS. Detection antibody: 84720-3-PBS. Standard: Eg1372. Range: 0.313-20 ng/mL.

JAM-A/CD321 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84720-1-PBS

Clone No.242290B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, JAM 1, JAM, F11 receptor

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Product Information

84720-1-PBS targets JAM-A/CD321 as part of a matched antibody pair:

MP00247-2: 84720-1-PBS capture and 84720-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: F11 receptor
• Calculated Molecular Weight: 33kDa
• GenBank Accession Number: NM_016946.6
• Gene Symbol: F11R
• Gene ID (NCBI): 50848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y624-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.