JAM-A/CD321 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HUVEC using 84720-2-RR (same clone as 84720-2-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84720-2-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 84720-2-RR (same clone as 84720-2-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84720-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00247-3

  Cytometric bead array standard curve of MP00247-3, F11R Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84720-2-PBS. Detection antibody: 84720-3-PBS. Standard: Eg1372. Range: 0.313-20 ng/mL.

JAM-A/CD321 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84720-2-PBS

Clone No.242290C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, JAM 1, JAM, F11 receptor

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Product Information

84720-2-PBS targets JAM-A/CD321 as part of a matched antibody pair:

MP00247-3: 84720-2-PBS capture and 84720-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: F11 receptor
• Calculated Molecular Weight: 33kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_016946.6
• Gene Symbol: F11R
• Gene ID (NCBI): 50848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y624-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.