JMJD4 Polyclonal antibody

JMJD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21572-1-AP

Synonyms

JMJD4, jumonji domain containing 4

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Validation Data Gallery

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  WB analysis of HeLa using 21572-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21572-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 21572-1-AP is JMJD4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JMJD4 fusion protein Ag16099
• Full Name: jumonji domain containing 4
• Calculated Molecular Weight: 463 aa, 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC109272
• Gene Symbol: JMJD4
• Gene ID (NCBI): 65094
• RRID: AB_2878886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JMJD4 (JmjC domain-containing protein 4, Jumonji domain-containing protein 4) is a member of the JmjC domain-containing proteins. Several JmjC domain-containing proteins have been reported to play a crucial role in the control of gene expression by acting as protein hydroxylases or histone demethylases (PMID: 16983801; 21300889). The function of JMJD4 protein remains unknown. The gene of JMJD4 maps to chromosome 1q42.13.

Publications

Species	Application	Title
human,mouse	WB	J Hematol Oncol; JMJD4-demethylated RIG-I prevents hepatic steatosis and carcinogenesis; Authors - Zhenyang Li
human	WB	Circulation; Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy; Authors - Yansong Tang ; KD Validated