JMJD5 Polyclonal antibody

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JMJD5 Polyclonal Antibody for WB, ELISA

Cat No. 20999-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

JMJD5, jumonji domain containing 5, KDM8, Lysine specific demethylase 8

• 

  WB analysis using 20999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20999-1-AP (JMJD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-453s cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20999-1-AP targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15115

  Product name: Recombinant human JMJD5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 67-416 aa of BC027911

  Sequence: SSEVILDYSWEKLNTGTWQDVDKDWRRVYAIGCLLKALCLCQAPEDANTVAAALRVCDMGLLMGAAILGDILLKVAAILQTHLPGKRPARGSLPEQPCTKKARADHGLIPDVKLEKTVPRLHRPSLQHFREQFLVPGRPVILKGVADHWPCMQKWSLEYIQEIAGCRTVPVEVGSRYTDEEWSQTLMTVNEFISKYIVNEPRDVGYLAQHQLFDQIPELKQDISIPDYCSLGDGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVMGRKYIRLYSPQESGALYPHDTHLLHNTSQVDVENPDLEKFPKFAKAPFLSCILSPGEILFIPVKYWHYVRALDLSFSVSFWWS

• Full Name: jumonji domain containing 5
• Calculated Molecular Weight: 416 aa, 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC027911
• Gene Symbol: JMJD5
• Gene ID (NCBI): 79831
• RRID: AB_3085630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N371
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JmjC (Jumonji-C) domain-containing 5 (JMJD5) (also called KDM8) is a 2-oxoglutarate (2-OG) dependent oxygenase of the JmjC subfamily. By shuttling between the nucleus and cytoplasm, JMJD5 could either associate with histones or cytosolic proteins, and thus plays an important role in embryonic development (PMID:22241836;22402282), circadian regulation (PMID:21115819), osteoclastogenesis (PMID:22375008), and stem-cell renewal (PMID:24740926).

Publications

What published studies show

The JMJD5 (KDM8) polyclonal antibody (Cat# 20999-1-AP) has 1 citation. It was used in a Western blot study published in Scientific Reports (2026), investigating how the KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes ovarian cancer progression. The research falls within the fields of epigenetics, cancer metabolism, and ovarian oncology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes the progression of ovarian cancer.; Authors - Chunyan Liu