JMJD7 Polyclonal antibody

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• JMJD7 Polyclonal antibody (21168-1-AP)

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  WB analysis using 21168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21168-1-AP (JMJD7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 21168-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21168-1-AP (JMJD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21168-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21168-1-AP (JMJD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 21168-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21168-1-AP (JMJD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 21168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 21168-1-AP (JMJD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 21168-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using JMJD7 antibody (21168-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

JMJD7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21168-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3S, Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7, EC:1.14.11.63, JmjC domain-containing protein 7, jumonji domain containing 7

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, U2OS cells
• Positive IHC detected in: human thyroid cancer tissue, human urothelial carcinoma tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21168-1-AP targets JMJD7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15371

  Product name: Recombinant human JMJD7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC139899

  Sequence: MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLPYFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELPQLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYELYTPATYQLTEEGTFKVVDEEAMEKVPWIPLDPLAPDLARYPSYSQAQALRCTVRAGEMLYLPALWFHHVQQSQGCIAVNFWYDMEYDLKYSYFQLLDSLTKASGLD

• Full Name: jumonji domain containing 7
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 35-40 kDa，60-70 kDa
• GenBank Accession Number: BC139899
• Gene Symbol: JMJD7
• Gene ID (NCBI): 100137047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P0C870
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JMJD 7 (Jumongji domain containing 7) is a protein containing JmjC domain, belonging to 2- ketoglutarate (2-OG)-dependent dioxygenase family. As an important epigenetic regulatory factor, JMJD7 plays a key role in many physiological and pathological processes such as gene transcription, cell metabolism and bone development (PMID: 41922800). Early biochemical and biophysical studies show that JMJD 7 has terminal endoprotease and aminopeptidase activities, and can target and cut the tail of histone with methylated arginine (such as H2, H3 or H4), thus participating in regulating the stability and transcription of nucleosomes. Abnormal expression of JMJD7 in various solid tumors. For example, it often fuses with phosphatase PLA2G4B, and participates in the development and progress of head and neck squamous cell carcinoma and other malignant tumors. It can also form homodimer (PMID: 29915238).