|Positive WB detected in||HeLa cells, mouse liver tissue, K-562 cells, rat liver tissue, HepG2 cells, NIH/3T3 cells, L02 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human testis tissue, human cerebellum tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10424-1-AP targets JTV1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||JTV1 fusion protein Ag0675|
|Full Name||JTV1 gene|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002853|
|Gene ID (NCBI)||7965|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1. There're two isoforms of JTV1 with MW about 27 kDa and 35 kDa,
|Product Specific Protocols|
|WB protocol for JTV1 antibody 10424-1-AP||Download protocol|
|IHC protocol for JTV1 antibody 10424-1-AP||Download protocol|
|IF protocol for JTV1 antibody 10424-1-AP||Download protocol|
|IP protocol for JTV1 antibody 10424-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Biol Chem
Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.
Fluorescence-Based Analysis of Noncanonical Functions of Aminoacyl-tRNA Synthetase-Interacting Multifunctional Proteins (AIMPs) in Peripheral Nerves.
Critical role of PINK1 in regulating Parkin protein levels in vivo.
Int J Oncol
Prostaglandin E2 promotes liver cancer cell growth by the upregulation of FUSE-binding protein 1 expression.
Dysregulation of parkin in the substantia nigra of db/db and high-fat diet mice.
Int J Mol Sci
CRISPR-Cas9 Mediated Telomere Removal Leads to Mitochondrial Stress and Protein Aggregation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
KRishnendu (Verified Customer) (02-27-2020)
Very strong antibody with no contaminant bands.