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Various lysates were subjected to SDS PAGE followed by western blot with RMX00032 (JUNB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Various lysates were subjected to SDS PAGE followed by western blot with RMX00032 (JUNB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IP experiment of C6 using RMX00032
IP result of anti-JUNB (IP:RMX00032, 4ug; Detection:RMX00032 1:10000) with C6 cells lysate 1960 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP result of anti-JUNB (IP:RMX00032, 4ug; Detection:RMX00032 1:10000) with C6 cells lysate 1960 ug.
IHC staining of human tonsillitis using RMX00032
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using RMX00032
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using RMX00032
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (RMX00032) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (RMX00032) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00032 targets JUNB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis using RMX00032
Various lysates were subjected to SDS PAGE followed by western blot with RMX00032 (JUNB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human tonsillitis using RMX00032
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using RMX00032
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using RMX00032 (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of C6 using RMX00032
IP result of anti-JUNB (IP:RMX00032, 4ug; Detection:RMX00032 1:10000) with C6 cells lysate 1960 ug.
IF/ICC Figures
IF Staining of HeLa using RMX00032
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (RMX00032) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
JUNB Multi-Recombinant antibody
Catalog Number
RMX00032
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
