Tested Applications
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51054-2-AP targets Jph2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0468 Product name: Recombinant mouse Jph2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 436-664 aa of NM_021566 Sequence: ILENSESLLEPPERGLGTGLPERPRESPQLHERETPQPEGGPPSPAGTPPQPKRPRPGASKDGLLSPGSWNGEPGGEGSRPATPSDGAGRRSPARPASEHMAIEALQPPPAPSQEPEVAMYRGYHSYAVRTGPPEPPPLEDEQEPEPEPEPEVRRSDSAPPSPVSATVPEEEPPAPRSPVPAKQATLEPKPIVPKAEPKAKARKTEARGLSKAGAKKKGRKEVAQAKEA Predict reactive species |
| Full Name | junctophilin 2 |
| Calculated Molecular Weight | 75 kDa 45 kDa |
| GenBank Accession Number | NM_021566 |
| Gene Symbol | Jph2 |
| Gene ID (NCBI) | 59091 |
| RRID | AB_717022 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ET78 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Jph2 antibody 51054-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Jph2 Polyclonal antibody (Cat# 51054-2-AP) has 1 total citation. It appears in bioRxiv, a preprint server. The cited study investigates Ca²⁺ influx through ER–plasma membrane contacts in brown fat thermogenesis and metabolic health, placing this citation in the fields of calcium signaling, lipid metabolism, and energy homeostasis. The application used was Western blot (WB) in mouse samples.
| Species | Application | Title |
|---|---|---|
bioRxiv Ca(2+) influx through ER-plasma membrane contacts is required for brown fat thermogenesis and metabolic health. |
