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  • KD/KO Validated

KARS Polyclonal antibody

KARS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14951-1-AP

Synonyms

KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, LysRS, lysyl tRNA synthetase



Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14951-1-AP targets KARS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KARS fusion protein Ag6778
Full Name lysyl-tRNA synthetase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC004132
Gene symbol KARS
Gene ID (NCBI) 3735
RRIDAB_2128158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KARS antibody 14951-1-APDownload protocol
IHC protocol for KARS antibody 14951-1-APDownload protocol
IF protocol for KARS antibody 14951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Sci Adv

Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.

Authors - J Guerra
zebrafishWB

Nucleic Acids Res

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Authors - Qinghai Zhang
humanWB

Nucleic Acids Res

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

Authors - Haissi Cui
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
humanWB

Hum Mutat

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Authors - Benedetta Ruzzenente
humanIF

Front Cell Infect Microbiol

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Authors - Tian Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)