• Featured Product
  • KD/KO Validated

KARS Polyclonal antibody

KARS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 14951-1-AP


KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, LysRS, lysyl tRNA synthetase

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14951-1-AP targets KARS in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KARS fusion protein Ag6778
Full Name lysyl-tRNA synthetase
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC004132
Gene Symbol KARS
Gene ID (NCBI) 3735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for KARS antibody 14951-1-APDownload protocol
IHC protocol for KARS antibody 14951-1-APDownload protocol
IF protocol for KARS antibody 14951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.

Authors - J Guerra

Nucleic Acids Res

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Authors - Qinghai Zhang

Nucleic Acids Res

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

Authors - Haissi Cui

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang

Hum Mutat

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Authors - Benedetta Ruzzenente

Front Cell Infect Microbiol

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Authors - Tian Luo


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)