KATNA1 Polyclonal antibody

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KATNA1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17560-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

p60 katanin, Katanin p60 subunit A1, Katanin p60 ATPase-containing subunit A1, EC:5.6.1.1

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  WB analysis using 17560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 17560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 17560-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 17560-1-AP

  IP result of anti-KATNA1 (IP:17560-1-AP, 4ug; Detection:17560-1-AP 1:500) with mouse brain tissue lysate 3440ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 17560-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17560-1-AP (KATNA1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HCT 116 cells, mouse brain tissue, rat brain tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17560-1-AP targets KATNA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11753

  Product name: Recombinant human KATNA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-311 aa of BC050428

  Sequence: MSLLMISENVKLAREYALLGNYDSAMVYYQGVLDQMNKYLYSVKDTYLQQKWQQVWQEINVEAKHVKDIMKTLESFKLDSTPLKAAQHDLPASEGEVWSMPVPVERRPSPGPRKRQSSQYSDPKSHGNRPSTTVRVHRSSAQNVHNDRGKAVRCREKKEQNKGREEKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSGMRP

• Full Name: katanin p60 (ATPase-containing) subunit A 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC050428
• Gene Symbol: KATNA1
• Gene ID (NCBI): 11104
• RRID: AB_10694670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75449
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

KATNA1 Polyclonal antibody (Cat# 17560-1-AP) has 16 citations spanning journals including Cell Research, Nature Cell Biology, Neuron, Developmental Cell, eLife, FASEB Journal, Journal of Biological Chemistry, and others. Research fields cover microtubule dynamics, neurite outgrowth, cilia biology, spindle architecture, cortical development, axon regeneration, and cytokinetic abscission. Cited applications include WB and IF in human, mouse, and rat samples.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Res; Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.; Authors - Lei Wang
human	WB	Nat Cell Biol; Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.; Authors - Kai Jiang
mouse	WB	Nat Cell Biol; Cell state-specific cytoplasmic density controls spindle architecture and scaling; Authors - Tobias Kletter
mouse	WB, IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
human	WB	Dev Cell; Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.; Authors - Kai Jiang
mouse	IF	PLoS Biol; Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.; Authors - Mary Mirvis